rabbit anti cholera toxin b subunit antibody  (Novus Biologicals)

Journal: NPJ Parkinson's Disease

Article Title: Calbindin and Girk2/Aldh1a1 define resilient vs vulnerable dopaminergic neurons in a primate Parkinson’s disease model

doi: 10.1038/s41531-024-00777-0

Figure Lengend Snippet: a Representative confocal images showing the expression of cholera toxin subunit B (CTB)-labeled midbrain neurons co-expressing Aldh1a1 after striatal tracer injection. White arrows show abundant Aldh1a1 + /CTB+ neurons. b Percentages of expression of Aldh1a1 + /CTB+ neurons in the monkey’s midbrain with injections in the caudate ( n = 2; 78 neurons) and putamen respectively ( n = 3; 623 neurons). c Representative confocal images showing the expression of CTB-labeled midbrain neurons expressing Aldh1a1 after globus pallidus injection. White arrows show abundant Aldh1a1-/CTB+ neurons. d Percentages of expression of Aldh1a1 + /CTB+ neurons in the monkey’s midbrain with injection in the external division if the globus pallidus (GPe) ( n = 1; 127 neurons). e , f Quantification of TH-immunoreactive axonal length in the GPe in control and MPTP-treated monkeys ( n = 2/group; MPTP n = 6). g Percentages of expression of Aldh1a1 + /CTB+ neurons in the monkey’s midbrain with injection in the internal division if the globus pallidus (GPi) ( n = 1; 139 neurons). h , i Quantification of TH-immunoreactive axonal length in the GPi in control and MPTP-treated monkeys ( n = 2/group; MPTP n = 6). Data represent mean + SEM. Ctrl, control; Asym, asymptomatic; Rec, recovered; Park, parkinsonian.

Article Snippet: Rabbit anti Cholera Toxin Subunit B , 1:1,000 , Sigma , C3062 , AB_258833.

Techniques: Expressing, Labeling, Injection, Control

Journal: NPJ Parkinson's Disease

Article Title: Calbindin and Girk2/Aldh1a1 define resilient vs vulnerable dopaminergic neurons in a primate Parkinson’s disease model

doi: 10.1038/s41531-024-00777-0

Figure Lengend Snippet: List of antibodies

Article Snippet: Rabbit anti Cholera Toxin Subunit B , 1:1,000 , Sigma , C3062 , AB_258833.

Techniques: Plasmid Preparation

Journal: PLoS ONE

Article Title: Low Cost Tuberculosis Vaccine Antigens in Capsules: Expression in Chloroplasts, Bio-Encapsulation, Stability and Functional Evaluation In Vitro

doi: 10.1371/journal.pone.0054708

Figure Lengend Snippet: Ls, Lactuca sativa ; Nt, Nicotiana tabacum ; pLD- vector components: the homologous Nicotiana tabacum chloroplast genome flanking sequence comprising of 16S 3′ end sequences and complete trn I, trn A genes; pLs- vector components: 16S trn I, the homologous Lactuca sativa chloroplast genome long flanking sequence containing 16S 3′end sequences and full length trn I gene; trn A 23S, trn A gene and 5′end of the 23S ribosomal RNA subunit; Prrn, ribosomal RNA operon promoter with GGAGG ribosome binding site; aad A, aminoglycoside 3′-adenylytransferase gene; TrbcL, 3′ untranslated region (Utr) of rbc L gene; PpsbA, promoter and 5′ Utr of psb A gene; TpsbA, 3′ Utr of psb A gene; CTB-ESAT6, coding sequence of cholera toxin B subunit fused to ESAT6 (6 kDa early secretory antigenic target); CTB-Mtb72F, coding sequence of cholera toxin B subunit fused to a recombinant fusion polyprotein from two TB antigens Mtb32 and Mtb39; LipY, coding sequence of a TB cell wall protein with lipase activity.

Article Snippet: Rabbit anti-cholera toxin B subunit polyclonal antibody (Abcam, ab34992) was coated on to washed protein A beads in a ratio of 50 µg/200 µl in 1X PBS overnight at 4°C for antibody binding to beads.

Techniques: Plasmid Preparation, Sequencing, Binding Assay, Recombinant, Activity Assay

Journal: Vaccines

Article Title: Characterization of ShigETEC, a Novel Live Attenuated Combined Vaccine against Shigellae and ETEC

doi: 10.3390/vaccines8040689

Figure Lengend Snippet: Toxin neutralizing capacity of mouse sera induced by ShigETEC vaccination. ( a ) Mice were vaccinated 3 times i.n. with 10 8 CFU ShigETEC. Serum at indicated dilutions was incubated with indicated amounts of LT, and LT-binding to GM1 coated plates was measured. Bound LT was detected with a polyclonal anti-cholera toxin antibody. ( b ) Sera from individual mice (3 times i.n. vaccinated with 10 8 CFU ShigETEC (blue symbols) or buffer (mock, black symbols) were pre-incubated with the 5 ng LT. LT induced cAMP release was measured in T84 human colon epithelial cells. ( c ) 5 ng synthetic ST was pre-incubated with pooled serum from mice vaccinated i.p. with LTB-ST N12S (blue bar) or LTB-ST WT (red bar) protein or vehicle (mock). ST-induced cGMP release was measured in T84 cells.

Article Snippet: Lysates and controls were incubated on plates, and bound LTB was detected with HRP-anti-cholera toxin B subunit (CTB) rabbit polyclonal antibody (Fisher Scientific) and ABTS substrate (Fisher Scientific).

Techniques: Incubation, Binding Assay